abstract：:Cell wall changes were examined in fruit of a melting flesh peach (Prunus persica L.) allowed to ripen on the tree. Three phases to softening were noted, the first of which began prior to the completion of flesh colour change and an increase in ethylene evolution. Softening in young mature fruit, prior to ripening, wa...

journal_title：Journal of experimental botany

authors： Brummell DA,Dal Cin V,Crisosto CH,Labavitch JM

更新日期：2004-09-01 00:00:00

abstract：:Partially tree-ripened ripe fruit of peach (Prunus persica L.) were stored for 1-4 weeks at 5 degrees C and then ripened at 20 degrees C for 3 d to induce chilling injury. With increasing cold storage the incidence and severity of mealiness symptoms increased progressively, manifested as reduced quantities of free jui...

journal_title：Journal of experimental botany

authors： Brummell DA,Dal Cin V,Lurie S,Crisosto CH,Labavitch JM

更新日期：2004-09-01 00:00:00

abstract：:Man's oldest fungicide has probably long functioned in this role in plants, as a natural component of induced antifungal defence. Elemental sulphur (S(0)) is the only inorganic phytoalexin and the only phytoalexin produced by so many different taxa. S(0) (detected by GC-MS as (32)S(8)) is produced in representative sp...

journal_title：Journal of experimental botany

authors： Cooper RM,Williams JS

更新日期：2004-08-01 00:00:00

abstract：:All members of the sulphotransferase (SOT, EC 2.8.2.-) protein family use 3'-phosphoadenosine 5'-phosphosulphate (PAPS) as the sulphuryl donor and transfer the sulphonate group to an appropriate hydroxyl group of several classes of substrates. These enzymes have highly conserved domains and can be found in eubacteria ...

journal_title：Journal of experimental botany

authors： Klein M,Papenbrock J

更新日期：2004-08-01 00:00:00

abstract：:Maize seedling water relations and abscisic acid (ABA) levels were measured over 24 h of root chilling (5.5 degrees C). At 2.5 h into chilling, leaf ABA levels increased by 40x and stomatal conductance (g(s)) decreased to 20% compared with prechill levels. Despite a rapid g(s) response to root chilling, leaf water pot...

journal_title：Journal of experimental botany

authors： Melkonian J,Yu LX,Setter TL

更新日期：2004-08-01 00:00:00

abstract：:In order to screen for putative candidate genes linked to tomato fruit weight and to sugar or acid content, genes and QTLs involved in fruit size and composition were mapped. Genes were selected among EST clones in the TIGR tomato EST database (http://www.tigr.org/tdb/tgi/lgi/) or corresponded to genes preferentially ...

journal_title：Journal of experimental botany

authors： Causse M,Duffe P,Gomez MC,Buret M,Damidaux R,Zamir D,Gur A,Chevalier C,Lemaire-Chamley M,Rothan C

更新日期：2004-08-01 00:00:00

abstract：:Plants require the function of plasma membrane-bound sulphate transporters for the initial uptake of inorganic sulphate. Part of this fundamental process is the energy-dependent proton/sulphate co-transport systems that are located in the surface cell layers of roots. During sulphur limitation, plants are able to acti...

journal_title：Journal of experimental botany

authors： Maruyama-Nakashita A,Nakamura Y,Yamaya T,Takahashi H

更新日期：2004-08-01 00:00:00

abstract：:The last steps of cysteine biosynthesis are catalysed by a bi-enzyme complex composed of serine acetyltransferase (SAT) and cysteine synthase, also called O-acetyl-serine (thiol) lyase (OASTL). SAT is responsible for the production of O-acetyl-serine (OAS) from serine and acetyl-coenzyme A, while OASTL catalyses the f...

journal_title：Journal of experimental botany

authors： Sirko A,Błaszczyk A,Liszewska F

更新日期：2004-08-01 00:00:00

abstract：:Among three AO isoforms detected in pea plants, the activity of PAO-1 was dominant in leaves of seedlings and young leaves of mature plants, while PAO-3 revealed the highest band intensity in old leaves and roots. PAO-1 and PAO-3 are homodimers consisting of 145 kDa and 140 kDa subunits, respectively, while PAO-2 is a...

journal_title：Journal of experimental botany

authors： Zdunek-Zastocka E,Omarov RT,Koshiba T,Lips HS

更新日期：2004-06-01 00:00:00

abstract：:Since cysteine is the first committed molecule in plant metabolism containing both sulphur and nitrogen, the regulation of its biosynthesis is critically important. Cysteine itself is required for the production of an abundance of key metabolites in diverse pathways. Plants alter their metabolism to compensate for sul...

journal_title：Journal of experimental botany

authors： Hesse H,Nikiforova V,Gakière B,Hoefgen R

更新日期：2004-06-01 00:00:00

abstract：:Transpiration of cuticular membranes isolated from the lower stomatous surface of Hedera helix (ivy) leaves was measured using a novel approach which allowed a distinction to be made between gas phase diffusion (through stomatal pores) and solid phase diffusion (transport through the polymer matrix membrane and cuticu...

journal_title：Journal of experimental botany

authors： Santrůcek J,Simánová E,Karbulková J,Simková M,Schreiber L

更新日期：2004-06-01 00:00:00

abstract：:The brownseed mutants (bs(1), bs(2), and bs(4)) of tomato all possess dark testae and deleteriously affect seed germination speed and/or final percentage. Poor germination performance of the bs(1) but not the bs(4) mutant, was due to greater impediment to radicle egress. Testa toughening (bs(1)) was prevented by dryin...

journal_title：Journal of experimental botany

authors： Downie AB,Dirk LM,Xu Q,Drake J,Zhang D,Dutt M,Butterfield A,Geneve RR,Corum JW 3rd,Lindstrom KG,Snyder JC

更新日期：2004-05-01 00:00:00

abstract：:Storage of phosphorus (P) in stem tissue is important in Mediterranean Proteaceae, because proteoid root growth and P uptake is greatest during winter, whereas shoot growth occurs mostly in summer. This has prompted the present investigation of the P distribution amongst roots, stems, and leaves of Hakea prostrata R.B...

journal_title：Journal of experimental botany

authors： Shane MW,McCully ME,Lambers H

更新日期：2004-05-01 00:00:00

abstract：:The effects of 24-epibrassinolide (EBR) spray application on gas-exchange, chlorophyll fluorescence characteristics, Rubisco activity, and carbohydrate metabolism were investigated in cucumber (Cucumis sativus L. cv. Jinchun No. 3) plants grown in a greenhouse. EBR significantly increased the light-saturated net CO(2)...

journal_title：Journal of experimental botany

authors： Yu JQ,Huang LF,Hu WH,Zhou YH,Mao WH,Ye SF,Nogués S

更新日期：2004-05-01 00:00:00

abstract：:The ability of two soybean (Glycine max L. [Merrill]) cultivars, 'Williams 82' and 'Maple Arrow', which were reported to use different ureide degradation pathways, to degrade the ureides allantoin and allantoate was investigated. Protein fractions and total leaf homogenates from the fourth trifoliate leaves of both cu...

journal_title：Journal of experimental botany

authors： Todd CD,Polacco JC

更新日期：2004-04-01 00:00:00

abstract：:In peach trees (Prunus persica L. Batsch cv. Redhaven), sorbitol is a primary photosynthetic product and may play an important role in the budbreak process. Surprisingly, before budbreak (from January to early March), the concentration of sorbitol in the xylem sap decreases, while that of hexoses (glucose and fructose...

journal_title：Journal of experimental botany

authors： Maurel K,Sakr S,Gerbe F,Guilliot A,Bonhomme M,Rageau R,Pétel G

更新日期：2004-04-01 00:00:00

abstract：:Plants of Lolium perenne L. were grown in sterile solution culture supplied with 2 mol m(-3) nitrogen as either nitrate or ammonium. Glutamine at 5 mol m(-3) was added to the nutrient solution of half the plants for 24 h. Root nitrate influx (at external nitrate concentrations 0-2000 mmol m(-3)) and amino acid concent...

journal_title：Journal of experimental botany

authors： Thornton B

更新日期：2004-03-01 00:00:00

abstract：:Ethylene production and expression of ethylene biosynthetic genes was investigated in senescing flowers of carnation (Dianthus caryophyllus L.) cultivars 'White Candle (WC)' and 'Light Pink Barbara (LPB)', with long and short vase-lives, respectively. Ethylene production from the gynoecium and petals of senescing 'WC'...

journal_title：Journal of experimental botany

authors： Nukui H,Kudo S,Yamashita A,Satoh S

更新日期：2004-03-01 00:00:00

abstract：:Plant voltage-gated channels belonging to the Shaker family participate in sustained K+ transport processes at the cell and whole plant levels, such as K+ uptake from the soil solution, long-distance K+ transport in the xylem and phloem, and K+ fluxes in guard cells during stomatal movements. The attention here is foc...

journal_title：Journal of experimental botany

authors： Chérel I

更新日期：2004-02-01 00:00:00

abstract：:High temperature stress (HTS), during flowering, decreases seed production in many plants. To determine the effect of a moderate HTS on flowering, fruit and seed set in Brassica napus, plants were exposed to a HTS (8/16 h dark/light, 18 degrees C night, ramped at 2 degrees C h-1, over 6 h, to 35 degrees C for 4 h, ram...

journal_title：Journal of experimental botany

authors： Young LW,Wilen RW,Bonham-Smith PC

更新日期：2004-02-01 00:00:00

abstract：:The protein encoded by the Arabidopsis At3g55360 gene was selected as a candidate for the enoyl reductase of the microsomal elongase system based on its homology to the Tsc13p protein of S. cerevisiae. The studies presented here demonstrate that heterologous expression of At3g55360 functionally complements the tempera...

journal_title：Journal of experimental botany

authors： Gable K,Garton S,Napier JA,Dunn TM

更新日期：2004-02-01 00:00:00

abstract：:Research into the molecular basis of plant-insect interactions is hampered by the inability to alter the expression of individual genes in plants growing under natural conditions. The ability of virus-induced gene silencing (VIGS) to silence the expression of two jasmonate-induced genes known to mediate the expression...

journal_title：Journal of experimental botany

authors： Saedler R,Baldwin IT

更新日期：2004-01-01 00:00:00

abstract：:The expression and activity of glutamine synthetase (GS, EC 6.3.1.2) were examined in relation to the rate of CO2 assimilation in sunflower (Helianthus annuus L.) leaves. Intact plants were kept in the dark for 72 h and subsequently exposed to light under different atmospheric CO2 concentrations (100, 400 and 1200 mic...

journal_title：Journal of experimental botany

authors： Larios B,Aguera E,Cabello P,Maldonado JM,de la Haba P

更新日期：2004-01-01 00:00:00

abstract：:Plants have evolved highly complex sensory mechanisms to monitor their surroundings and adapt their growth and development to the prevailing environmental conditions. The integration of information from multiple environmental cues enables the co-ordination of development with favourable seasonal conditions and, ultima...

journal_title：Journal of experimental botany

authors： Franklin KA,Whitelam GC

更新日期：2004-01-01 00:00:00

abstract：:The present work describes the intrachloroplast localization and the changes that took place in the thylakoid and stroma-located superoxide dismutases (SOD, EC 1.15.1.1) and ascorbate peroxidases (APX, EC 1.11.1.11), in response to long-term NaCl stress in Pisum sativum L. cv. Puget plants. Native PAGE using high chlo...

journal_title：Journal of experimental botany

authors： Gomez JM,Jimenez A,Olmos E,Sevilla F

更新日期：2004-01-01 00:00:00

abstract：:Brassinosteroids (BRs) are steroidal phytohormones that are essential for many processes in plant growth and development, such as cell expansion, vascular differentiation, and responses to stress. The effects of BRs on cell division are unclear, as attested by contradictory published results. To determine the effect o...

journal_title：Journal of experimental botany

authors： Miyazawa Y,Nakajima N,Abe T,Sakai A,Fujioka S,Kawano S,Kuroiwa T,Yoshida S

更新日期：2003-12-01 00:00:00

abstract：:Modifications to the cell wall of developing and ripening tomato fruit are mediated by cell wall-degrading enzymes, including a beta-d-xylosidase or alpha-l-arabinofuranosidase, which participate in the breakdown of xylans and/or arabinoxylans. The activity of both enzymes was highest during early fruit growth, before...

journal_title：Journal of experimental botany

authors： Itai A,Ishihara K,Bewley JD

更新日期：2003-12-01 00:00:00

abstract：:The composition of the translocates reaching the seeds of pea plants having various nitrogen (N) nutrition regimes was investigated under field situations. Sucrose flow in the phloem sap increased with the node number, but was not significantly different between N nutrition levels. Because N deficiency reduced the num...

journal_title：Journal of experimental botany

authors： Munier-Jolain N,Salon C

更新日期：2003-11-01 00:00:00

abstract：:Low-temperature stress is an important factor affecting the growth and development of rice (Oryza sativa L.) in temperate and high-elevation areas. Cold stress may cause various seedling injuries, delayed heading and yield reduction due to spikelet sterility. In this study, 181 microsatellite marker loci were used to ...

journal_title：Journal of experimental botany

authors： Andaya VC,Mackill DJ

更新日期：2003-11-01 00:00:00

abstract：:Tomato (Lycopersicon esculentum) plants were grown in liquid culture inside the greenhouse of Hiroshima University, Japan. At the first fruiting stage, P was withdrawn from the rooting medium for a period of 19 d and its effect was studied on photosynthesis, stomatal conductance, transpiration, partitioning of 13C and...

journal_title：Journal of experimental botany

authors： Fujita K,Okada M,Lei K,Ito J,Ohkura K,Adu-Gyamfi JJ,Mohapatra PK

更新日期：2003-11-01 00:00:00

abstract：:A new method for the isolation of root hairs from the model legume, Medicago truncatula, was developed. The procedure involves the propagation of detached roots on agar plates and the collection of root hairs by immersion in liquid nitrogen. Yields of up to 40 micro g of root hair protein were obtained from 50-100 roo...

journal_title：Journal of experimental botany

authors： Ramos J,Bisseling T

更新日期：2003-10-01 00:00:00

abstract：:The kinetics of leaf vein recovery from cavitation-induced embolism was studied in plants of sunflower cv. Margot, together with the impact of vein embolism on the overall leaf hydraulic conductance (Kleaf). During the air-dehydration of leaves to leaf water potentials (Psi L) of -1.25 MPa, Kleaf was found to decrease...

journal_title：Journal of experimental botany

authors： Trifilò P,Gascó A,Raimondo F,Nardini A,Salleo S

更新日期：2003-10-01 00:00:00

abstract：:The activity of ionically bound peroxidases from an asparagus spear increased from 5-24 h post-harvest. Isoelectric focusing showed that the post-harvest increase of the total peroxidase activity was due to the increase of several distinct isoperoxidases. Concomitantly, a decrease in the activity of two anionic peroxi...

journal_title：Journal of experimental botany

authors： Holm KB,Andreasen PH,Eckloff RM,Kristensen BK,Rasmussen SK

更新日期：2003-10-01 00:00:00

abstract：:The root cap assists the passage of the root through soil by means of its slimy mucilage secretion and by the sloughing of its outer cells. The root penetration resistance of decapped primary roots of maize (Zea mays L. cv. Mephisto) was compared with that of intact roots in loose (dry bulk density 1.0 g cm-3; penetra...

journal_title：Journal of experimental botany

authors： Iijima M,Higuchi T,Barlow PW,Bengough AG

更新日期：2003-09-01 00:00:00

abstract：:Cellulose synthases (CesA) represent a group of beta-1,4 glycosyltransferases involved in cellulose biosynthesis. Recent molecular studies confirmed that a large CesA gene family encoding various CesA isoforms exists in plants. The isolation and characterization of a new, full-length CesA cDNA, PtrCesA5 from an econom...

journal_title：Journal of experimental botany

authors： Kalluri UC,Joshi CP

更新日期：2003-09-01 00:00:00

abstract：:Constitutive over-expression of a maize sucrose-phosphate synthase (SPS) gene in tobacco (Nicotiana tabacum) had major effects on leaf carbohydrate budgets with consequences for whole plant development. Transgenic tobacco plants flowered earlier and had greater flower numbers than wild-type plants. These changes were ...

journal_title：Journal of experimental botany

authors： Baxter CJ,Foyer CH,Turner J,Rolfe SA,Quick WP

更新日期：2003-08-01 00:00:00

abstract：:An investigation was carried out to assess the effect of nitrate supply on the root plasma membrane (PM) H+-ATPase of etiolated maize (Zea mays L.) seedlings grown in hydroponics. The treatment induced higher uptake rates of the anion and the expression of a putative high-affinity nitrate transporter gene (ZmNRT2.1), ...

journal_title：Journal of experimental botany

authors： Santi S,Locci G,Monte R,Pinton R,Varanini Z

更新日期：2003-08-01 00:00:00

abstract：:Nitrogen (N) nutrition, post-anthesis temperature and drought-induced changes in the kinetics of accumulation of dry mass, total grain N and protein fractions (albumins-globulins, amphiphils, gliadins, and glutenins) contents were examined for winter wheat (Triticum aestivum L.). Crops were grown in controlled environ...

journal_title：Journal of experimental botany

authors： Triboï E,Martre P,Triboï-Blondel AM

更新日期：2003-07-01 00:00:00

abstract：:A cDNA clone encoding aspartate aminotransferase (PVAAT-2) (EC 2.6.1.1) was isolated from the common bean Phaseolus vulgaris nodule cDNA library. The nucleotide sequence analysis of the full-length cDNA allowed its identification by comparison with sequence databases. The amino acid sequence of the bean PvAAT-2 showed...

journal_title：Journal of experimental botany

authors： Silvente S,Camas A,Lara M

更新日期：2003-06-01 00:00:00

abstract：:PaHB1 (for Picea abies Homeobox1), an evolutionarily conserved HD-GL2 homeobox gene, specifically expressed in the protoderm during somatic embryogenesis in the gymnosperm Norway spruce has been reported previously. An additional HD-GL2 gene designated PaHB2 is reported here. During somatic embryogenesis, the PaHB2 ge...

journal_title：Journal of experimental botany

authors： Ingouff M,Farbos I,Wiweger M,von Arnold S

更新日期：2003-05-01 00:00:00